Vol. 60 No. 2 (2023): including 12th Special issue on Grapevine Trunk Diseases
Short Notes - 12th Special issue on Grapevine Trunk Diseases

Dual labelled probe assays for differentiation of Botryosphaeria dothidea, Neofusicoccum mediterraneum and Neofusicoccum parvum, based on polymorphisms in the MAT1-2-1 gene

Stavros PALAVOUZIS
Laboratory of Phytopathology, Department of Crop Science, School of Plant Sciences, Agricultural University of Athens
Bio
Alexandra TRIANTAFYLLOPOULOU
Laboratory of Phytopathology, Department of Crop Science, School of Plant Sciences, Agricultural University of Athens
Bio
Christos TSOUKAS
Laboratory of Phytopathology, Department of Crop Science, School of Plant Sciences, Agricultural University of Athens
Bio
Aliki K. TZIMA
Laboratory of Phytopathology, Department of Crop Science, School of Plant Sciences, Agricultural University of Athens
Bio
Epaminondas PAPLOMATAS
Laboratory of Phytopathology, Department of Crop Science, School of Plant Sciences, Agricultural University of Athens
Bio

Published 2023-07-23

Keywords

  • diagnosis,
  • mating type genes,
  • molecular marker,
  • real time PCR

How to Cite

[1]
S. PALAVOUZIS, A. TRIANTAFYLLOPOULOU, C. TSOUKAS, A. K. TZIMA, and E. PAPLOMATAS, “Dual labelled probe assays for differentiation of Botryosphaeria dothidea, Neofusicoccum mediterraneum and Neofusicoccum parvum, based on polymorphisms in the MAT1-2-1 gene”, Phytopathol. Mediterr., vol. 60, no. 2, pp. 177–182, Jul. 2023.

Funding data

Abstract

Botryosphaeriaceous fungi are widespread, and cause serious diseases in many economically important crops. Botryosphaeria dothidea, Neofusicoccum mediterraneum and N. parvum are the most important members of this family in the Mediterranean region. These fungi are frequently isolated from the same host, which together with their extensive and increasing host range necessitates development of rapid and reliable diagnostic tools. Species boundaries within the Botryosphaeriaceae have been defined based on phylogenetic analyses of multiple gene sequences, including those of mating type genes. The MAT1-2-1 gene displayed high sequence variability between Botryosphaeriaceous species, so was selected as the target for development of a definitive diagnostic tool. This paper outlines a new and robust molecular tool, composed of three TaqMan assays based on polymorphisms located in the MAT1-2-1 gene of B. dothidea, N. mediterraneum and N. parvum. Each assay differentiated the target species from other Botryosphaeriaceae, and from non-target fungi.

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