Vol. 60 No. 2 (2023): including 12th Special issue on Grapevine Trunk Diseases
Research papers - 12th Special Issue on Grapevine Trunk Diseases

In vitro evaluation of drying supports and adhesive polymers as adjuvants for biocontrol of Diplodia seriata by Trichoderma harzianum and Clonostachys rosea

Katherina AGUIRRE
Laboratorio de Fitopatología y Control Biológico de Enfermedades, Departamento de Sanidad Vegetal, Facultad de Ciencias Agronómicas, Universidad de Chile. Casilla 1004, Santiago
Laboratorio de Fitopatología y Control Biológico de Enfermedades, Departamento de Sanidad Vegetal, Facultad de Ciencias Agronómicas, Universidad de Chile. Casilla 1004, Santiago
Laboratorio de Fitopatología y Control Biológico de Enfermedades, Departamento de Sanidad Vegetal, Facultad de Ciencias Agronómicas, Universidad de Chile. Casilla 1004, Santiago

Published 2023-09-15


  • Adjuvants,
  • bioformulation,
  • Botryosphaeria,
  • drying supports,
  • sticky polymers

How to Cite

K. AGUIRRE, L. M. PEREZ, and J. R. MONTEALEGRE, “In vitro evaluation of drying supports and adhesive polymers as adjuvants for biocontrol of Diplodia seriata by Trichoderma harzianum and Clonostachys rosea”, Phytopathol. Mediterr., vol. 60, no. 2, pp. 227–237, Sep. 2023.


Grapevine trunk diseases (GTDs) can cause large losses in vineyards. Diplodia seriata is an important GTD pathogen in Chile. Development and use of bioprotectors is a complementary alternative to the use of agrochemicals for disease management. To produce bioformulations for management of D. seriata, additives could be used to maintain viability and survival of biocontrol agents, such as Trichoderma harzianum and Clonostachys rosea. Effects of drying supports (inulin, maltodextrin, lactose, or talc) and adhesive polymers (carboxymethylcellulose, Aloe vera gel, or chitosan) were assessed on D. seriata conidium viability and mycelium development of T. harzianum and C. rosea, and for their biocontrol capacity against D. seriata. T. harzianum and C. rosea cultured in Potato Dextrose Agar containing inulin (at 10% w/v) maltodextrin (10% w/v), lactose (6% w/v), or talc (4% w/v), or the adhesive polymers carboxymethylcellulose (0.5% w/v), Aloe vera gel (0.5% w/v), or chitosan (1.5% w/v), maintained their biocontrol activity against D. seriata. These additives did not enhance D. seriata development. Therefore, these preparations, at the respective indicated concentrations, can be included in bioformulations for management of disease caused by this pathogen.


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