Research papers - 12th Special Issue on Grapevine Trunk Diseases
In vitro evaluation of drying supports and adhesive polymers as adjuvants for biocontrol of Diplodia seriata by Trichoderma harzianum and Clonostachys rosea
Katherina AGUIRRE- Luz M. PEREZ
- Jaime R. MONTEALEGRE
Categories
Published 2023-09-15
Keywords
- Adjuvants,
- bioformulation,
- Botryosphaeria,
- drying supports,
- sticky polymers
How to Cite
[1]
K. AGUIRRE, L. M. PEREZ, and J. R. MONTEALEGRE, “In vitro evaluation of drying supports and adhesive polymers as adjuvants for biocontrol of Diplodia seriata by Trichoderma harzianum and Clonostachys rosea”, Phytopathol. Mediterr., vol. 62, no. 2, pp. 227–237, Sep. 2023.
Copyright (c) 2023 Katherina AGUIRRE, Luz M. PEREZ, Jaime R. MONTEALEGRE
This work is licensed under a Creative Commons Attribution 4.0 International License.
Abstract
Grapevine trunk diseases (GTDs) can cause large losses in vineyards. Diplodia seriata is an important GTD pathogen in Chile. Development and use of bioprotectors is a complementary alternative to the use of agrochemicals for disease management. To produce bioformulations for management of D. seriata, additives could be used to maintain viability and survival of biocontrol agents, such as Trichoderma harzianum and Clonostachys rosea. Effects of drying supports (inulin, maltodextrin, lactose, or talc) and adhesive polymers (carboxymethylcellulose, Aloe vera gel, or chitosan) were assessed on D. seriata conidium viability and mycelium development of T. harzianum and C. rosea, and for their biocontrol capacity against D. seriata. T. harzianum and C. rosea cultured in Potato Dextrose Agar containing inulin (at 10% w/v) maltodextrin (10% w/v), lactose (6% w/v), or talc (4% w/v), or the adhesive polymers carboxymethylcellulose (0.5% w/v), Aloe vera gel (0.5% w/v), or chitosan (1.5% w/v), maintained their biocontrol activity against D. seriata. These additives did not enhance D. seriata development. Therefore, these preparations, at the respective indicated concentrations, can be included in bioformulations for management of disease caused by this pathogen.
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References
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Du J., Ge Z., Xu Z., Zou B., Zhang Y., Li C., 2014. Comparison of the efficiency of five different drying carriers on the spray drying of persimmon pulp powders. Drying Technology 32(10): 1157–1166. https://doi.org/10.1080/07373937.2014.886259
El-Mohamedy R., Abdel-Kareem F., Jabnoun-Khiareddine H., DaamiRemadi M., 2014. Chitosan and Trichoderma harzianum as fungicide alternatives for controlling Fusarium crown and root rot of tomato. Tunisian Journal of Plant Protection 9: 31-43.
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Fravel D., 2005. Commercialization and implementation of biocontrol. Annual Review of Phytopathology 43: 337-359.
https://doi.org/10.1146/annurev.phyto.43.032904.092924
Gašić S., Tanović B., 2013. Biopesticide formulations, possibility of application and future trends. Journal Pesticides and Phytomedicine 28(2): 97-102. https://doi.org/10.2298/PIF1302097G
Gramaje D., Urbez-Torres J., Sosnowski M., 2018. Managing Grapevine Trunk Diseases with Respect to Etiology and Epidemiology: Current Strategies and Future Prospects. Plant Disease 102: 12-39. https://doi.org/10.1094/PDIS-04-17-0512-FE
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Higl B., Kurtmann L., Carlsen C.U., Ratjen J., Först P., … Risbo J., 2008. Impact of water activity, temperature, and physical state on the storage stability of Lactobacillus paracasei ssp paracasei freeze‐dried in a lactose matrix. Biotechnology progress 23(4): 794-800. https://doi.org/10.1021/bp070089d
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Jensen B., Knudsen I., Jensen, D.F., 2000. Biological seed treatment of cereals with fresh and long-term stored formulations of Clonostachys rosea: Biocontrol efficacy against Fusarium culmorum. European Journal of Plant Pathology 106(3): 233-242. https://doi.org/10.1023/A:1008794626600
Jensen B., Knudsen I., Madsen M., Jensen D., 2004. Biopriming of infected carrot seed with an antagonist, Clonostachys rosea, selected for control of seedborne Alternaria spp. Phytopathology 94: 551-560. https://doi.org/10.1094/PHYTO.2004.94.6.551
Kakvan N., Heydari A., Zamanizadeh H., Rezaee S., Naraghi L., 2013. Development of new bioformulations using Trichoderma and Talaromyces fungal antagonists for biological control of sugar beet damping-off disease. Crop Protection 53: 80-84. https://doi.org/10.1016/j.cropro.2013.06.009
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Khamna S., Yokota A., 2008. Actinomycetes isolated from medicinal plant rhizosphere soils: diversity and screening of antifungal compounds, indole-3-aceticacid and siderophore production. World Journal of Microbiology and Biotechnology 25: 649-655. https://doi.org/10.1007/s11274-008-9933-x
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