Articles
Characterization of Colletotrichum strains associated with olive anthracnose in Sicily
Grazia LICCIARDELLO- Juan MORAL
- Maria Concetta STRANO
- Paola CARUSO
- Patrizia BELLA
- Silvia DI SILVESTRO
Published 2022-05-13
Keywords
- Pathogenicity,
- multilocus analyses,
- cultivar susceptibility,
- phylogenetic tree
How to Cite
[1]
G. LICCIARDELLO, “Characterization of Colletotrichum strains associated with olive anthracnose in Sicily”, Phytopathol. Mediterr., vol. 61, no. 1, pp. 139–151, May 2022.
Funding data
-
Ministero delle Politiche Agricole Alimentari e Forestali
Grant numbers DM 59 del 10/01/2018
Abstract
Anthracnose caused by Colletotrichum spp. is the most damaging olive fruit disease in many countries, including Italy. This disease has been sporadically detected in Sicily, but new agronomic practices can increase risk of olive anthracnose in this region. An etiological study of the disease focused on local olive cultivars growing at the International Olive Germplasm Collection (IOGC) in Villa Zagaria, Enna, Sicily has been undertaken. During 2018 and 2019, 137 Colletotrichum strains were isolated from olives. Colony morphology, conidium characteristics, and multilocus sequence analyses aided identification of three species: C. acutatum (affecting 70% of symptomatic olives), C. gloeosporioides, and C. cigarro. Three C. acutatum strains (B13-16, P77, and P185), and one strain of each C. gloeosporioides (C2.1) and C. cigarro (Perg6B) were evaluated for pathogenicity on olive fruits from 11 Sicilian cultivars, known for their high-quality oil. Differences in virulence were detected among strains and their pathogenicity to the cultivars. The C. acutatum isolates were more virulent than those of C. gloeosporioides or C. cigarro. The Sicilian olive cultivars Cavaliera, Carolea, Calatina, and Nocellara del Belice were the most susceptible to the pathogen, while the cultivars Biancolilla and Nocellara Etnea were the most tolerant. Cultivar response under field conditions showed that anthracnose severity and fruit-rot incidence were positively correlated. This is the first report of C. acutatum and C. cigarro affecting olive trees in Sicily. Control measures for anthracnose depend on accurate characterization of the etiological agents and host cultivar resistance.
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References
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Agosteo G.E., Magnano di San Lio G., Cacciola S.O., Frisullo S., 2002. Characterisation of the causal agent of olive Anthracnose in Southern Italy. Acta Horticulturae 586: 713–716.
Aiello D., Carrieri R., Guarnaccia V., Vitale A., Lahoz E., Polizzi, G., 2015. Characterization and pathogenicity of Colletotrichum gloeosporioides and C. karstii causing preharvest disease on Citrus sinensis in Italy. Journal of Phytopathology 163:168–177.
Baroncelli R., Talhinhas P., Pensec F., Sukno S.A., Le Floch G., Thon, M., 2017. The Colletotrichum acutatum species complex as a model system to study evolution and host specialization in plant pathogens. Frontiers in Microbiology 8: 2001.
Cabral A., Azinheira H.G., Talhinhas P., Batista D., Ramos A.P., Silva M.D.C., Oliveira H., Várzea V., 2020. Pathological, morphological, cytogenomic, biochemical and molecular data support the distinction between Colletotrichum cigarro comb. et stat. nov. and Colletotrichum kahawae. Plants 9: 502.
Cacciola S.O., Faedda R., Sinatra F., Agosteo G.E., Schena L., … Magnano di San Lio G., 2012. Olive Anthracnose. Journal of Plant Pathology 94: 29–44.
Campbell, C. L., and Madden, L. V., 1990. Introduction to plant disease epidemiology. John Wiley & Sons.
Cannon P.F., Damm U., Johnston P.R., Weir B.S., 2012. Colletotrichum –current status and future directions. Studies in Mycology 73: 181–213.
Chattaoui M., Raya M.C., Bouri M., Moral J., Perez-Rodriguez M.,…Rhouma A., 2016). Characterization of a Colletotrichum population causing Anthracnose disease on olive in northern Tunisia. Journal of Applied Microbiology 120: 1368–1381.
Damm U., Cannon P.F., Woundenberg J.H.C., Crous P.W., 2012. The Colletotrichum acutatum species complex. Studies in Mycology 73: 27–113.
Embaby E.M., Haggag L.F., Abdel-Monem M.O., El-Sayed T.I., Al-Awam L.R., 2014. Anthracnose disease (Colletotrichum sp.) affecting olive fruit quality and its control in Egypt. Journal of Agricultural Technology 10: 1289-1306.
Faedda R., Agosteo G.E., Schena L., Mosca S., Frisullo S., …Cacciola S.O., 2011. Colletotrichum clavatum sp. nov. identified as the causal agent of olive Anthracnose in Italy. Phytopathologia Mediterranea 50: 283–302.
Graniti A., 1954. La “lebbra” delle olive in Sicilia. Olivicoltura 9: 1-5.
Guarnaccia V., Groenewald J.Z., Polizzi G., Crous, P.W., 2017 High species diversity in Colletotrichum associated with citrus diseases in Europe. Persoonia 39: 32–50.
Guerber J.C., Liu B., Correll J.C., Johnston P.R., 2003. Characterization of diversity in Colletotrichum acutatum sensu lato by sequence analysis of two gene introns, mtDNA and intron RFLPs, and mating compatibility. Mycologia 95: 872–895.
Iliadi M.K., Tjamos E.C., Antoniou P.P., Tsitsigiannis D.I., 2018. First report of Colletotrichum acutatum causing Anthracnose on olives in Greece. Plant Disease 102: 820.
Ismail A.M., Cirvilleri G., Yaseen T., Epifani F., Perrone G., Polizzi G., 2015. Characterisation of Colletotrichum species causing Anthracnose disease of mango in Italy. Journal of Plant Pathology 97: 167–171.
Kumar S., Stecher G., Li M., Knyaz C., Tamura K., 2018. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Molecular Biology and Evolution 35: 1547–1549.
Moral J., Bouhmidi K., Trapero A., 2008. Influence of fruit maturity, cultivar susceptibilitity, and inoculation method on infection of olive fruit by Colletotrichum acutatum. Plant Disease 92: 1421–1426.
Moral J., Oliveira R., Trapero A., 2009. Elucidation of the disease cycle of olive Anthracnose caused by Colletotrichum acutatum. Phytopathology 99: 548–556.
Moral J., Trapero A., 2012. Mummified fruit as a source of inoculum and disease dynamics of olive Anthracnose caused by Colletotrichum spp. Phytopathology 102: 982–989.
Moral J., Xaviér C., Roca L.F., Romero J., Moreda W., Trapero A., 2014. La Antracnosis del olivo y su efecto en la calidad del aceite. Grasas y Aceites 65: e028.
Moral J., Xaviér C.J., Viruega J.R., Roca L.F., Caballero J., Trapero A., 2017. Variability in Susceptibility to Anthracnose in the World Collection of Olive Cultivars of Cordoba (Spain). Frontiers in Plant Science 8: 1892.
Moral J., Agustí-Brisach C., Raya M.C., Jurado-Bello J., López-Moral, A., …Trapero, A., 2021. Diversity of Colletotrichum species associated with olive Anthracnose worldwide. Journal of Fungi 7: 741.
Mosca S., Li Destri Nicosia M.G., Cacciola S.O., Schena L., 2014. Molecular analysis of Colletotrichum species in the carposphere and phyllosphere of olive. PLoS One 9: e114031.
Msairi S., Chliyeh M., Rhimini Y., Selmaoui K., Mouria A., Touhami A. O., Benkirane R., Douira A., 2017. First Report on Colletotrichum acutatum of Olives in Morocco. Annual Research and Review in Biology: 16: 1-8.
Muzzalupo I., Chiappetta A., Stabile G., Bucci C., Perri E., 2011. Intra-varietal difference of “Carolea” olive assessed by molecular markers. Acta Horticulture 918: 771-776.
Peres F., Talhinhas P., Afonso H., Alegre H., Oliveira H., Ferreira-Dias S., 2021. Olive oils from fruits infected with different Anthracnose pathogens show sensory defects earlier than chemical degradation. Agronomy 11, 1041.
Perrone G., Magistà D., Ismail A.M., 2016. First report of Colletotrichum kahawae subsp. ciggaro on mandarin in Italy. Journal of Plant Pathology 98: 12.
Polizzi G., Aiello D., Guarnaccia V., Vitale A., Perrone, G., Stea G., 2011. First report of damping-off on strawberry tree caused by Colletotrichum acutatum and C. simmondsii in Italy. Plant Disease 95: 1588.
Rhouma A., Triki M.A., Msallem M., 2010. First report of olive Anthracnose caused by Colletotrichum gloeosporioides in Tunisia. Phytopathologia Mediterranea 49: 95–98.
Sanei S.J., Razavi S.E, 2012. Survey of olive fungal disease in north of Iran. Annual Review and Research in Biology 2: 27–36.
Schena L., Mosca S., Cacciola S., Faedda R., Sanzani S., … Magnano di San Lio G., 2014. Species of the Colletotrichum gloeosporioides and C. boninense complexes associated with olive Anthracnose. Plant Pathology 63: 437– 446.
Schena L., Abdelfattah A., Mosca S., Li Destri Nicosia M.G., Agosteo G.E., Cacciola S.O., 2017. Quantitative detection of Colletotrichum godetiae and C. acutatum sensu stricto in the phyllosphere and carposphere of olive during four phenological phases. European Journal of Plant Pathology 149: 337–347.
Talhinhas P., Sreenivasaprasad S., Neves-Martins J., Oliveira, H., 2005. Molecular and phenotypic analyses reveal the association of diverse Colletotrichum acutatum groups and a low level of C. gloeosporioides with olive Anthracnose. Applied and Environmental Microbiology 71: 2987–2998.
Talhinhas P., Neves-Martins J., Oliveira H., Sreenivasaprasad S., 2009. The distinctive population structure of Colletotrichum species associated with olive Anthracnose in the Algarve region of Portugal reflects a host-pathogen diversity hot spot. FEMS Microbiology Letters 296: 31–38.
Talhinhas P., Mota-Capitao C., Martins S., Ramos A.P., Neves‐Martins J., Oliveira H., 2011 Epidemiology, histopathology and aetiology of olive Anthracnose caused by Colletotrichum acutatum and C. gloeosporioides in Portugal. Plant Pathology 60: 483–495.
Talhinhas P., Loureiro A., Oliveira H., 2018. Olive Anthracnose: a yield‐and oil quality‐degrading disease caused by several species of Colletotrichum that differ in virulence, host preference and geographical distribution. Molecular Plant Pathology 19: 1797-1807.
Talhinhas P., Baroncelli R., 2021. Colletotrichum species and complexes: geographic distribution, host range and conservation status. Fungal Diversity. https://doi.org/10.1007/s13225-021-00491-9
Weir B.S., Johnston P.R., Damm U., 2012. The Colletotrichum gloeosporioides species complex. Studies in Mycology 73: 115–80.
Zar J.H., 2010. Biostatistical Analysis. 5th ed. Pearson Education India, New Delhi, India, 944 pp.
Agosteo G.E., Magnano di San Lio G., Cacciola S.O., Frisullo S., 2002. Characterisation of the causal agent of olive Anthracnose in Southern Italy. Acta Horticulturae 586: 713–716.
Aiello D., Carrieri R., Guarnaccia V., Vitale A., Lahoz E., Polizzi, G., 2015. Characterization and pathogenicity of Colletotrichum gloeosporioides and C. karstii causing preharvest disease on Citrus sinensis in Italy. Journal of Phytopathology 163:168–177.
Baroncelli R., Talhinhas P., Pensec F., Sukno S.A., Le Floch G., Thon, M., 2017. The Colletotrichum acutatum species complex as a model system to study evolution and host specialization in plant pathogens. Frontiers in Microbiology 8: 2001.
Cabral A., Azinheira H.G., Talhinhas P., Batista D., Ramos A.P., Silva M.D.C., Oliveira H., Várzea V., 2020. Pathological, morphological, cytogenomic, biochemical and molecular data support the distinction between Colletotrichum cigarro comb. et stat. nov. and Colletotrichum kahawae. Plants 9: 502.
Cacciola S.O., Faedda R., Sinatra F., Agosteo G.E., Schena L., … Magnano di San Lio G., 2012. Olive Anthracnose. Journal of Plant Pathology 94: 29–44.
Campbell, C. L., and Madden, L. V., 1990. Introduction to plant disease epidemiology. John Wiley & Sons.
Cannon P.F., Damm U., Johnston P.R., Weir B.S., 2012. Colletotrichum –current status and future directions. Studies in Mycology 73: 181–213.
Chattaoui M., Raya M.C., Bouri M., Moral J., Perez-Rodriguez M.,…Rhouma A., 2016). Characterization of a Colletotrichum population causing Anthracnose disease on olive in northern Tunisia. Journal of Applied Microbiology 120: 1368–1381.
Damm U., Cannon P.F., Woundenberg J.H.C., Crous P.W., 2012. The Colletotrichum acutatum species complex. Studies in Mycology 73: 27–113.
Embaby E.M., Haggag L.F., Abdel-Monem M.O., El-Sayed T.I., Al-Awam L.R., 2014. Anthracnose disease (Colletotrichum sp.) affecting olive fruit quality and its control in Egypt. Journal of Agricultural Technology 10: 1289-1306.
Faedda R., Agosteo G.E., Schena L., Mosca S., Frisullo S., …Cacciola S.O., 2011. Colletotrichum clavatum sp. nov. identified as the causal agent of olive Anthracnose in Italy. Phytopathologia Mediterranea 50: 283–302.
Graniti A., 1954. La “lebbra” delle olive in Sicilia. Olivicoltura 9: 1-5.
Guarnaccia V., Groenewald J.Z., Polizzi G., Crous, P.W., 2017 High species diversity in Colletotrichum associated with citrus diseases in Europe. Persoonia 39: 32–50.
Guerber J.C., Liu B., Correll J.C., Johnston P.R., 2003. Characterization of diversity in Colletotrichum acutatum sensu lato by sequence analysis of two gene introns, mtDNA and intron RFLPs, and mating compatibility. Mycologia 95: 872–895.
Iliadi M.K., Tjamos E.C., Antoniou P.P., Tsitsigiannis D.I., 2018. First report of Colletotrichum acutatum causing Anthracnose on olives in Greece. Plant Disease 102: 820.
Ismail A.M., Cirvilleri G., Yaseen T., Epifani F., Perrone G., Polizzi G., 2015. Characterisation of Colletotrichum species causing Anthracnose disease of mango in Italy. Journal of Plant Pathology 97: 167–171.
Kumar S., Stecher G., Li M., Knyaz C., Tamura K., 2018. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Molecular Biology and Evolution 35: 1547–1549.
Moral J., Bouhmidi K., Trapero A., 2008. Influence of fruit maturity, cultivar susceptibilitity, and inoculation method on infection of olive fruit by Colletotrichum acutatum. Plant Disease 92: 1421–1426.
Moral J., Oliveira R., Trapero A., 2009. Elucidation of the disease cycle of olive Anthracnose caused by Colletotrichum acutatum. Phytopathology 99: 548–556.
Moral J., Trapero A., 2012. Mummified fruit as a source of inoculum and disease dynamics of olive Anthracnose caused by Colletotrichum spp. Phytopathology 102: 982–989.
Moral J., Xaviér C., Roca L.F., Romero J., Moreda W., Trapero A., 2014. La Antracnosis del olivo y su efecto en la calidad del aceite. Grasas y Aceites 65: e028.
Moral J., Xaviér C.J., Viruega J.R., Roca L.F., Caballero J., Trapero A., 2017. Variability in Susceptibility to Anthracnose in the World Collection of Olive Cultivars of Cordoba (Spain). Frontiers in Plant Science 8: 1892.
Moral J., Agustí-Brisach C., Raya M.C., Jurado-Bello J., López-Moral, A., …Trapero, A., 2021. Diversity of Colletotrichum species associated with olive Anthracnose worldwide. Journal of Fungi 7: 741.
Mosca S., Li Destri Nicosia M.G., Cacciola S.O., Schena L., 2014. Molecular analysis of Colletotrichum species in the carposphere and phyllosphere of olive. PLoS One 9: e114031.
Msairi S., Chliyeh M., Rhimini Y., Selmaoui K., Mouria A., Touhami A. O., Benkirane R., Douira A., 2017. First Report on Colletotrichum acutatum of Olives in Morocco. Annual Research and Review in Biology: 16: 1-8.
Muzzalupo I., Chiappetta A., Stabile G., Bucci C., Perri E., 2011. Intra-varietal difference of “Carolea” olive assessed by molecular markers. Acta Horticulture 918: 771-776.
Peres F., Talhinhas P., Afonso H., Alegre H., Oliveira H., Ferreira-Dias S., 2021. Olive oils from fruits infected with different Anthracnose pathogens show sensory defects earlier than chemical degradation. Agronomy 11, 1041.
Perrone G., Magistà D., Ismail A.M., 2016. First report of Colletotrichum kahawae subsp. ciggaro on mandarin in Italy. Journal of Plant Pathology 98: 12.
Polizzi G., Aiello D., Guarnaccia V., Vitale A., Perrone, G., Stea G., 2011. First report of damping-off on strawberry tree caused by Colletotrichum acutatum and C. simmondsii in Italy. Plant Disease 95: 1588.
Rhouma A., Triki M.A., Msallem M., 2010. First report of olive Anthracnose caused by Colletotrichum gloeosporioides in Tunisia. Phytopathologia Mediterranea 49: 95–98.
Sanei S.J., Razavi S.E, 2012. Survey of olive fungal disease in north of Iran. Annual Review and Research in Biology 2: 27–36.
Schena L., Mosca S., Cacciola S., Faedda R., Sanzani S., … Magnano di San Lio G., 2014. Species of the Colletotrichum gloeosporioides and C. boninense complexes associated with olive Anthracnose. Plant Pathology 63: 437– 446.
Schena L., Abdelfattah A., Mosca S., Li Destri Nicosia M.G., Agosteo G.E., Cacciola S.O., 2017. Quantitative detection of Colletotrichum godetiae and C. acutatum sensu stricto in the phyllosphere and carposphere of olive during four phenological phases. European Journal of Plant Pathology 149: 337–347.
Talhinhas P., Sreenivasaprasad S., Neves-Martins J., Oliveira, H., 2005. Molecular and phenotypic analyses reveal the association of diverse Colletotrichum acutatum groups and a low level of C. gloeosporioides with olive Anthracnose. Applied and Environmental Microbiology 71: 2987–2998.
Talhinhas P., Neves-Martins J., Oliveira H., Sreenivasaprasad S., 2009. The distinctive population structure of Colletotrichum species associated with olive Anthracnose in the Algarve region of Portugal reflects a host-pathogen diversity hot spot. FEMS Microbiology Letters 296: 31–38.
Talhinhas P., Mota-Capitao C., Martins S., Ramos A.P., Neves‐Martins J., Oliveira H., 2011 Epidemiology, histopathology and aetiology of olive Anthracnose caused by Colletotrichum acutatum and C. gloeosporioides in Portugal. Plant Pathology 60: 483–495.
Talhinhas P., Loureiro A., Oliveira H., 2018. Olive Anthracnose: a yield‐and oil quality‐degrading disease caused by several species of Colletotrichum that differ in virulence, host preference and geographical distribution. Molecular Plant Pathology 19: 1797-1807.
Talhinhas P., Baroncelli R., 2021. Colletotrichum species and complexes: geographic distribution, host range and conservation status. Fungal Diversity. https://doi.org/10.1007/s13225-021-00491-9
Weir B.S., Johnston P.R., Damm U., 2012. The Colletotrichum gloeosporioides species complex. Studies in Mycology 73: 115–80.
Zar J.H., 2010. Biostatistical Analysis. 5th ed. Pearson Education India, New Delhi, India, 944 pp.