Vol. 61 No. 1 (2022)
Articles

Rutin-stevioside and related conjugates for potential control of grapevine trunk diseases

Laura Buzón-Durán
Department of Agricultural and Forestry Engineering, ETSIIAA, Universidad de Valladolid, Avda. Madrid 44, 34004 Palencia
Natalia Langa-Lomba
Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), EPS, Universidad de Zaragoza, Carretera de Cuarte s/n, 22071 Huesca
Vicente González-García
Plant Protection Unit, Agrifood Research and Technology Centre of Aragón, Instituto Agroalimentario de Aragón—IA2 (CITA-Universidad de Zaragoza), Avda. Montañana 930, 50059 Zaragoza
José Casanova-Gascón
Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), EPS, Universidad de Zaragoza, Carretera de Cuarte s/n, 22071 Huesca
Eva Sánchez-Hernández
Department of Agricultural and Forestry Engineering, ETSIIAA, Universidad de Valladolid, Avda. Madrid 44, 34004 Palencia
Jesús Martín-Gil
Department of Agricultural and Forestry Engineering, ETSIIAA, Universidad de Valladolid, Avda. Madrid 44, 34004 Palencia
Pablo Martín Ramos
Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), EPS, Universidad de Zaragoza, Carretera de Cuarte s/n, 22071 Huesca
Published March 25, 2022
Keywords
  • Antifungal,
  • Botryosphaeria dieback,
  • candyleaf,
  • GTDs,
  • rutoside
How to Cite
[1]
L. Buzón-Durán, “Rutin-stevioside and related conjugates for potential control of grapevine trunk diseases”, Phytopathol. Mediterr., vol. 61, no. 1, pp. 65-77, Mar. 2022.

Funding data

  • Junta de Castilla y León
    Grant numbers VA258P18
  • Universidad de Zaragoza
    Grant numbers Convocatoria Fundación Ibercaja-Universidad de Zaragoza de proyectos de investigación, desarrollo e innovación para jóvenes investigadores
  • Universidad de Zaragoza
    Grant numbers IV Convocatoria de Ayudas de la Cátedra AgroBank para la transferencia del conocimiento al sector agroalimentario

Abstract

Flavonoids and phenolic acids play roles in grapevine defence against pathogens causing grapevine trunk diseases (GTDs). Rutin is a major flavonoid in vegetative organs of the grapevines, and this compound, unlike other flavonoids, is non-toxic and non-oxidizable. Rutin was assayed in vitro and in vivo against two Botryosphaeriaceae taxa. The limited bioavailability of this compound was circumvented by conjugation with stevioside, a glycoside obtained from Stevia rebaudiana. Clear synergistic effects were observed for the stevioside-rutin adduct, resulting in EC50 and EC90 values of 306.0 and 714.9 μg·mL-1 against Neofusicoccum parvum and 241.6 and 457.8 μg·mL-1 against Dothiorella viticola. In greenhouse experiments, moderate inhibition of N. parvum growth and complete inhibition of D. viticola were observed. These inhibitory effects were greater than those of ferulic acid, which has been considered the most effective phenolic acid against GTDs. Conjugation with stevioside provided solubility enhancement of rutin, paving the way to the design of glycopesticides based on rutin-rich plant extracts as promising antifungals against GTDs.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...