Articles
Nightshade (Solanum nigrum), an intermediate host between tomato and cucurbits of Tomato leaf curl New Delhi virus
Published 2021-11-15
Keywords
- Begomovirus,
- genetic diversity,
- leaf curl
How to Cite
[1]
M. Ansar, “Nightshade (Solanum nigrum), an intermediate host between tomato and cucurbits of Tomato leaf curl New Delhi virus”, Phytopathol. Mediterr., vol. 60, no. 3, pp. 409–419, Nov. 2021.
Abstract
Geminiviruses infect many crop plants, and are limiting factors for vegetable crop production. Begomoviruses (Geminiviridae) cause typical symptoms of leaf curling and puckering in nightshade (Solanum nigrum), a seasonal weed in Bihar, India. To investigate if nightshade was an intermediate host for begomovirus, virus DNA was extracted and characterized. The DNA-A of the virus yielded 2737 nt and DNA-B yielded 2706 nt. The intergenic region (IR) showed a conserved nonanucleotide sequence that potentially forms a stem-loop structure. The genomic sequence of DNA-A shared 94% identity with that of Tomato leaf curl New Delhi virus (ToLCNDV)-ivy gourd isolate. However, the sequence of DNA-B showed 95% identity with a bitter gourd isolate. PCR-based detection revealed the presence ToLCNDV in bottle gourd, pumpkin, sponge gourd, and bitter gourd. The IR sequences of the viruses isolated from these cucurbits and tomato were 100% identical. Whitefly-mediated transmission of the virus to cucurbits and tomato from nightshade was also demonstrated. These results indicate that nightshade may act as reservoir of ToLCNDV, and is involved in developing epidemics in cucurbit species. The strain of ToLCNDV has probably adapted from solanaceous to cucurbitaceous hosts. This is the first report of ToLCNDV infecting nightshade in India, highlighting this virus as a possible cause of disease epidemics in economically important cucurbits.
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References
Agnihotri A.K., Mishra S.P., Tripathi R.C., Ansar M., Srivastava A., Tripathi I.P., 2018. First natural co-occurrence of tomato leaf curl New Delhi virus DNA-A and chili leaf curl betasatellite on tomato plants (Solanum lycopersicum L.) in India. Journal of General Plant Pathology 84: 414–417.
Agnihotri A.K., Mishra S.P., Ansar M., Tripathi R.C., Singh R., Akram M., 2019 Molecular Characterization of Mungbean yellow mosaic India virus infecting tomato (Solanum lycopersicum L.). Australasian Plant Pathology 48: 159-165.
Ansar M., Akram M., Agnihotri A.K., Srinivasaraghavan A., Saha T., Naimuddin., 2018. Characterization of leaf curl virus in chili and overwintering role of nightshade in linkage between chili and tomato. Journal of Plant Pathology 101: 307-314.
Ansar M., Agnihotri A.K., Akram M., Bhagat A.P., 2019. First report of Tomato leaf curl Joydebpur virus infecting French bean (Phaseolus vulgaris L.). Journal of General Plant Pathology 85: 444-448.
Briddon R.W., Bull S.E., Mansoor S., Amin I., Markham P.G. 2002. Universal primers for the PCR-mediated amplification of DNA β. Molecular Biotechnology 20:315–318.
Deng A., Mcgrath P.F., Robinson D.J., Harrison B.D., 1994. Detection and differentiation of whitefly transmitted geminiviruses in plants and vector insects by the polymerase chain reaction with degenerate primers. Annals of Applied Biology 125: 327–336.
Fontes E.P., Gladfelter H.J., Schaffer R.L., Petty I.T., Hanley-Bowdoin L. 1994. Geminivirus replication origins have a modular organization. Plant Cell 6: 405–416.
Fortes I.M., Sánchez-Campos S., Fiallo-Olivé E., Díaz-Pendón A.J., Navas-Castillo J., Moriones E., 2016. A novel strain of tomato leaf curl New Delhi virus has spread to the mediterranean basin. Viruses 8: 307.
Haider M.S., Tahir M., Latif S., Briddon R.W., 2005. First report of Tomato leaf curl New Delhi virus infecting Ecliptaprostrata in Pakistan. New Disease Report 11: 39.
Hanley-Bowdoin L., Bejarano E.R., Robertson D., Mansoor S., 2013. Geminiviruses: Masters at redirecting and reprogramming plant processes. Nature Review Microbiology 11: 777–788.
Hussain M., Mansoor S., Iram S., Zafar Y., Briddon R.W., 2004. First report of tomato leaf curl New Delhi virus affecting chilli pepper in Pakistan. Plant Pathology 53: 794.
Ito T., Sharma P., Kittipakorn K., Ikegami M., 2008. Complete nucleotide sequence of a new isolate of tomato leaf curl New Delhi virus infecting cucumber, bottle gourd and muskmelon in Thailand. Archives of Virology 153: 611–613.
Inoue-Nagata A.K., Lima M.F., Gilbertson R.L. 2016. A review of geminivirus diseases in vegetables and other crops in Brazil: current status and approaches for management. Horticultura Brasileira 34: 008-018.
Jyothsna P., Haq Q.M.I., Singh P., Sumiya K.V., Praveen S.,… Malathi V.G., 2013. Infection of tomato leaf curl New Delhi virus (ToLCNDV), a bipartite begomovirus with betasatellites, results in enhanced level of helper virus components and antagonistic interaction between DNA B and betasatellites. Applied Microbiology and Biotechnology 97: 5457–5471.
Khan M.S., Raj S.K., Singh R., 2006. First report of tomato leaf curl New Delhi virus infecting chilli in India. Plant Pathology 55: 289.
Kumari P., Singh A.K., Chattopadhyay B., Chakraborty S., 2010. Molecular characterization of a new species of Begomovirus and betasatellite causing leaf curl disease of tomato in India. Virus Research 152: 19–29.
Martin D.P., Lemey P., Lott M., Moulton V., Posada D., Lefeuvre P. 2010. RDP3: a flexible and fast computer program for analyzing recombination. Bioinformatics 26: 2462–2463.
Mnari-Hattab M., Zammouri S., Belkadhi M., Doña D.B., Ben Nahia E., Hajlaoui M., 2015. First report of tomato leaf curl New Delhi virus infecting cucurbits in Tunisia. New Disease Report 31: 21.
Moriones E., Praveen S., Chakraborty S., 2017. Tomato Leaf Curl New Delhi Virus: An emerging virus complex threatening vegetable and fibre crops. Viruses 9: 264.
Mubin M., Briddon R.W., Mansoor S., 2009. Diverse and recombinant DNA betasatellites are associated with a begomovirus disease complex of Digeraarvensis, a weed host. Virus Research 142: 208-212.
Mubin M., Shahid M. S., Tahir M. N., Briddon R. W., Mansoor S., 2010. Characterization of begomovirus components from a weed suggests that begomoviruses may associate with multiple distinct DNA satellites. Virus Genes. 40: 452-457.
Muniyappa V., Venkatesh H.M., Ramappa H.K., Kulkarni R.S., Zeidan M.,… Czosnek H., 2000. Tomato leaf curl virus from Bangalore (ToLCV-Ban4): sequence comparison with Indian ToLCV isolates, detection in plants and insects, and vector relationships. Archives of Virology 145: 1583–1598.
Naimuddin K., Akram M., Aniruddha A.K., 2016. Molecular characterization of a first begomovirus associated with lentil (Lens culinaris) from India. Acta Virologica 60 (3): 217-223.
Padidam M., Beachy R.N., Fauquet C.M., 1995. Tomato leaf curl geminivirus from India has a bipartite genome and coat protein is not essential for infectivity. Journal of General Virology 76: 25–35.
Panno S., Iacono G., Davino M., Marchione S., Zappardo V., … Davino S., 2016. First report of tomato leaf curl New Delhi virus affecting zucchini squash in an important horticultural area of southern Italy. New Disease Report 33: 6.
Papayiannis L.C., Katis N.I., Idris A.M., Brown J.K., 2011. Identification of weed hosts of Tomato yellow leaf curl virus in Cyprus. Plant Disease 95: 120-125.
Rojas M R., Gilbertson R.L., Russell D.R., Maxwell D.P., 1993. Use of degenerate primers in the polymerase chain reaction to detect whitefly transmitted geminiviruses. Plant Disease 77: 340-347.
Seal S.E., Jeger M.J., Vanden B.F., Begomovirus evolution and disease management. Advances in Virus Research 67: 297–316.
Senanayake D.M.J.B., Mandal B., Lodha S., Varma A., 2007. First report of Chilli leaf curl virus affecting chilli in India. Plant Pathology 56: 343.
Singh A.K., Chattopadhyay B., Chakraborty S., 2012. Biology and interactions of two distinct monopartite begomoviruses and betasatellites associated with radish leaf curl disease in India. Virology Journal 9: 43.
Tamura K., Strecher G., Peterson D., Filipski A., Kumar S., 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30: 2725–2739.
Wyant P.S., Gotthardt D., Schafer B., Krenz B., Jeske H., 2011. The genomes of four novel begomoviruses and a new Sidamicrantha mosaic virus strain from Bolivian weeds. Arch of Virology 156: 347-352.
Yazdani-Khameneh S., Aboutorabi S., Shoori M., Aghazadeh A., Jahanshahi P.,… Maleki M., 2016. Natural occurrence of tomato leaf curl New Delhi virus in Iranian cucurbit crops. Plant Pathology Journal 32: 201–208.
Zaidi S.S., Shakir S., Malik H.J., Farooq M., Amin I., Mansoor S., 2007. First report of Tomato leaf curl New Delhi virus on Calotropisprocera, a weed as potential reservoir Begomovirus host in Pakistan. Plant Disease 101(6): 1071-1072.
Zehra S.B., Ahmad A., Sharma A., Sofi S., Lateef A., … Rathore J.P., 2017. Chilli leaf curl virus an emerging threat to chilli in India. International Journal of Pure Applied Biosciences 5 (5): 404–414.
Zerbini F.M., Briddon R.W., Idris A., Martin D.P., Moriones E., … Varsani A., 2017. ICTV Virus taxonomy profile: Geminiviridae. Journal of General Virology 98: 131–133.
Agnihotri A.K., Mishra S.P., Ansar M., Tripathi R.C., Singh R., Akram M., 2019 Molecular Characterization of Mungbean yellow mosaic India virus infecting tomato (Solanum lycopersicum L.). Australasian Plant Pathology 48: 159-165.
Ansar M., Akram M., Agnihotri A.K., Srinivasaraghavan A., Saha T., Naimuddin., 2018. Characterization of leaf curl virus in chili and overwintering role of nightshade in linkage between chili and tomato. Journal of Plant Pathology 101: 307-314.
Ansar M., Agnihotri A.K., Akram M., Bhagat A.P., 2019. First report of Tomato leaf curl Joydebpur virus infecting French bean (Phaseolus vulgaris L.). Journal of General Plant Pathology 85: 444-448.
Briddon R.W., Bull S.E., Mansoor S., Amin I., Markham P.G. 2002. Universal primers for the PCR-mediated amplification of DNA β. Molecular Biotechnology 20:315–318.
Deng A., Mcgrath P.F., Robinson D.J., Harrison B.D., 1994. Detection and differentiation of whitefly transmitted geminiviruses in plants and vector insects by the polymerase chain reaction with degenerate primers. Annals of Applied Biology 125: 327–336.
Fontes E.P., Gladfelter H.J., Schaffer R.L., Petty I.T., Hanley-Bowdoin L. 1994. Geminivirus replication origins have a modular organization. Plant Cell 6: 405–416.
Fortes I.M., Sánchez-Campos S., Fiallo-Olivé E., Díaz-Pendón A.J., Navas-Castillo J., Moriones E., 2016. A novel strain of tomato leaf curl New Delhi virus has spread to the mediterranean basin. Viruses 8: 307.
Haider M.S., Tahir M., Latif S., Briddon R.W., 2005. First report of Tomato leaf curl New Delhi virus infecting Ecliptaprostrata in Pakistan. New Disease Report 11: 39.
Hanley-Bowdoin L., Bejarano E.R., Robertson D., Mansoor S., 2013. Geminiviruses: Masters at redirecting and reprogramming plant processes. Nature Review Microbiology 11: 777–788.
Hussain M., Mansoor S., Iram S., Zafar Y., Briddon R.W., 2004. First report of tomato leaf curl New Delhi virus affecting chilli pepper in Pakistan. Plant Pathology 53: 794.
Ito T., Sharma P., Kittipakorn K., Ikegami M., 2008. Complete nucleotide sequence of a new isolate of tomato leaf curl New Delhi virus infecting cucumber, bottle gourd and muskmelon in Thailand. Archives of Virology 153: 611–613.
Inoue-Nagata A.K., Lima M.F., Gilbertson R.L. 2016. A review of geminivirus diseases in vegetables and other crops in Brazil: current status and approaches for management. Horticultura Brasileira 34: 008-018.
Jyothsna P., Haq Q.M.I., Singh P., Sumiya K.V., Praveen S.,… Malathi V.G., 2013. Infection of tomato leaf curl New Delhi virus (ToLCNDV), a bipartite begomovirus with betasatellites, results in enhanced level of helper virus components and antagonistic interaction between DNA B and betasatellites. Applied Microbiology and Biotechnology 97: 5457–5471.
Khan M.S., Raj S.K., Singh R., 2006. First report of tomato leaf curl New Delhi virus infecting chilli in India. Plant Pathology 55: 289.
Kumari P., Singh A.K., Chattopadhyay B., Chakraborty S., 2010. Molecular characterization of a new species of Begomovirus and betasatellite causing leaf curl disease of tomato in India. Virus Research 152: 19–29.
Martin D.P., Lemey P., Lott M., Moulton V., Posada D., Lefeuvre P. 2010. RDP3: a flexible and fast computer program for analyzing recombination. Bioinformatics 26: 2462–2463.
Mnari-Hattab M., Zammouri S., Belkadhi M., Doña D.B., Ben Nahia E., Hajlaoui M., 2015. First report of tomato leaf curl New Delhi virus infecting cucurbits in Tunisia. New Disease Report 31: 21.
Moriones E., Praveen S., Chakraborty S., 2017. Tomato Leaf Curl New Delhi Virus: An emerging virus complex threatening vegetable and fibre crops. Viruses 9: 264.
Mubin M., Briddon R.W., Mansoor S., 2009. Diverse and recombinant DNA betasatellites are associated with a begomovirus disease complex of Digeraarvensis, a weed host. Virus Research 142: 208-212.
Mubin M., Shahid M. S., Tahir M. N., Briddon R. W., Mansoor S., 2010. Characterization of begomovirus components from a weed suggests that begomoviruses may associate with multiple distinct DNA satellites. Virus Genes. 40: 452-457.
Muniyappa V., Venkatesh H.M., Ramappa H.K., Kulkarni R.S., Zeidan M.,… Czosnek H., 2000. Tomato leaf curl virus from Bangalore (ToLCV-Ban4): sequence comparison with Indian ToLCV isolates, detection in plants and insects, and vector relationships. Archives of Virology 145: 1583–1598.
Naimuddin K., Akram M., Aniruddha A.K., 2016. Molecular characterization of a first begomovirus associated with lentil (Lens culinaris) from India. Acta Virologica 60 (3): 217-223.
Padidam M., Beachy R.N., Fauquet C.M., 1995. Tomato leaf curl geminivirus from India has a bipartite genome and coat protein is not essential for infectivity. Journal of General Virology 76: 25–35.
Panno S., Iacono G., Davino M., Marchione S., Zappardo V., … Davino S., 2016. First report of tomato leaf curl New Delhi virus affecting zucchini squash in an important horticultural area of southern Italy. New Disease Report 33: 6.
Papayiannis L.C., Katis N.I., Idris A.M., Brown J.K., 2011. Identification of weed hosts of Tomato yellow leaf curl virus in Cyprus. Plant Disease 95: 120-125.
Rojas M R., Gilbertson R.L., Russell D.R., Maxwell D.P., 1993. Use of degenerate primers in the polymerase chain reaction to detect whitefly transmitted geminiviruses. Plant Disease 77: 340-347.
Seal S.E., Jeger M.J., Vanden B.F., Begomovirus evolution and disease management. Advances in Virus Research 67: 297–316.
Senanayake D.M.J.B., Mandal B., Lodha S., Varma A., 2007. First report of Chilli leaf curl virus affecting chilli in India. Plant Pathology 56: 343.
Singh A.K., Chattopadhyay B., Chakraborty S., 2012. Biology and interactions of two distinct monopartite begomoviruses and betasatellites associated with radish leaf curl disease in India. Virology Journal 9: 43.
Tamura K., Strecher G., Peterson D., Filipski A., Kumar S., 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30: 2725–2739.
Wyant P.S., Gotthardt D., Schafer B., Krenz B., Jeske H., 2011. The genomes of four novel begomoviruses and a new Sidamicrantha mosaic virus strain from Bolivian weeds. Arch of Virology 156: 347-352.
Yazdani-Khameneh S., Aboutorabi S., Shoori M., Aghazadeh A., Jahanshahi P.,… Maleki M., 2016. Natural occurrence of tomato leaf curl New Delhi virus in Iranian cucurbit crops. Plant Pathology Journal 32: 201–208.
Zaidi S.S., Shakir S., Malik H.J., Farooq M., Amin I., Mansoor S., 2007. First report of Tomato leaf curl New Delhi virus on Calotropisprocera, a weed as potential reservoir Begomovirus host in Pakistan. Plant Disease 101(6): 1071-1072.
Zehra S.B., Ahmad A., Sharma A., Sofi S., Lateef A., … Rathore J.P., 2017. Chilli leaf curl virus an emerging threat to chilli in India. International Journal of Pure Applied Biosciences 5 (5): 404–414.
Zerbini F.M., Briddon R.W., Idris A., Martin D.P., Moriones E., … Varsani A., 2017. ICTV Virus taxonomy profile: Geminiviridae. Journal of General Virology 98: 131–133.