Vol. 60 No. 3 (2021)
Articles

Nightshade (Solanum nigrum), an intermediate host between tomato and cucurbits of Tomato leaf curl New Delhi virus

Mohammad Ansar
Department of Plant Pathology, Bihar Agricultural University, Sabour-813 210, Bhagalpur, Bihar
Aniruddha Kumar Agnihotri
Division of Crop Protection, Indian Institute of Pulses Research, Kanpur-208 024, U.P.
Tushar Ranjan
Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour-813 210 Bhagalpur, Bihar
Monika Karn
Department of Plant Pathology, Dr. Y S Parmar University of Horticulture and Forestry, Nauni Solan-173 230, Himanchal Pradesh
Srinivasaraghavan A
Department of Plant Pathology, Bihar Agricultural University, Sabour-813 210, Bhagalpur, Bihar
Ravi Ranjan Kumar
Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour-813 210 Bhagalpur, Bihar
Arun Prasad Bhagat
Department of Plant Pathology, Bihar Agricultural University, Sabour-813 210, Bhagalpur, Bihar
Published November 15, 2021
Keywords
  • Begomovirus,
  • genetic diversity,
  • leaf curl
How to Cite
[1]
M. Ansar, “Nightshade (Solanum nigrum), an intermediate host between tomato and cucurbits of Tomato leaf curl New Delhi virus”, Phytopathol. Mediterr., vol. 60, no. 3, pp. 409-419, Nov. 2021.

Abstract

Geminiviruses infect many crop plants, and are limiting factors for vegetable crop production. Begomoviruses (Geminiviridae) cause typical symptoms of leaf curling and puckering in nightshade (Solanum nigrum), a seasonal weed in Bihar, India. To investigate if nightshade was an intermediate host for begomovirus, virus DNA was extracted and characterized. The DNA-A of the virus yielded 2737 nt and DNA-B yielded 2706 nt. The intergenic region (IR) showed a conserved nonanucleotide sequence that potentially forms a stem-loop structure. The genomic sequence of DNA-A shared 94% identity with that of Tomato leaf curl New Delhi virus (ToLCNDV)-ivy gourd isolate. However, the sequence of DNA-B showed 95% identity with a bitter gourd isolate. PCR-based detection revealed the presence ToLCNDV in bottle gourd, pumpkin, sponge gourd, and bitter gourd. The IR sequences of the viruses isolated from these cucurbits and tomato were 100% identical. Whitefly-mediated transmission of the virus to cucurbits and tomato from nightshade was also demonstrated. These results indicate that nightshade may act as reservoir of ToLCNDV, and is involved in developing epidemics in cucurbit species. The strain of ToLCNDV has probably adapted from solanaceous to cucurbitaceous hosts. This is the first report of ToLCNDV infecting nightshade in India, highlighting this virus as a possible cause of disease epidemics in economically important cucurbits.

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