Articles
Published 2021-09-13
Keywords
- Acremonium cucurbitacearum,
- cucurbits,
- disease,
- identification,
- pathogen
- Plectosphaerella melonis,
- soilborne ...More
How to Cite
[1]
E. KOPILOV, H. TSEKHMISTER, O. NADKERNYCHNA, and A. KYSLYNSKA, “Identification of Plectosphaerella melonis from cucumber plants in Ukraine”, Phytopathol. Mediterr., vol. 60, no. 2, pp. 259–263, Sep. 2021.
Abstract
A fungus was isolated from diseased roots of Cucumis sativus grown in greenhouses. The morphological and cultural characteristics of the isolate allowed it to be classified as Plectosphaerella melonis. BLASTn analysis revealed 99% homology of the ITS sequence from the isolate with 14 Acremonium cucurbitacearum and P. melonis isolates, allowing attribution of the isolate to P. melonis (syn. A. cucurbitacearum). Koch’s hypothesis requirements were fulfilled for the isolate. Symptoms on host roots developed after 14 d of growing cucumber plants on infested soil. Plants of the cucumber variety Nizhynskyi 12 were very susceptible at the two leaf growth stage (2 weeks after sowing). Above-ground disease symptoms were absent after 14 d, even with severely diseased roots. This is the first report of P. melonis on C. sativus in Ukraine.
Downloads
Download data is not yet available.
Metrics
Metrics Loading ...
References
Aegerter B.J., Gordon T.R., Davis R.M., 2000. Occurrence and pathogenicity of fungi associated with melon root rot and vine decline in California. Plant Disease. 84 (3): 224–230.
Alfaro-Fernández A., García-Luis A., 2009. Colonisation and histological changes in muskmelon and autumn squash tissues infected by Acremonium cucurbitacearum or Monosporascus cannonballus. European Journal of Plant Pathology 125 (1): 73–85.
Alfaro-García A., Armengol J., Bruton B.D., Gams W., García-Jiménez J., Martínez-Ferrer G., 1996. The taxonomic position of the causal agent of Acremonium collapse. Mycologia 88: 804–808.
Armengol J., 1997. Aspectos patológicos, epidemiológicos y culturales de Acremonium cucurbitacearum Alfaro-Garcia, W. Gams et J. Garcia-Jiménez. PhD thesis. Universidad Politecnica de Valencia, Valencia, Spain.
Armengol J., Sanz E., Martínez‐Ferrer G., Sales R., Bruton B.D., García‐Jiménez J., 1998. Host range of Acremonium cucurbitacearum, cause of Acremonium collapse of muskmelon. Plant Pathology 47: 29–35.
Biernaki M., Bruton B.D., 2001. Quantitative response of Cucumis melo inoculated with root rot pathogens. Plant Disease 85: 65–70.
Birnboim H.C., Doly J. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7(6): 1513–1523. doi: 10.1093/nar/7.6.1513
Bondarenko S.A., Georgieva M.L., Bilanenko E.N., 2016. Alkalitolerant micromycetes in acidic and neutral soils of the temperate zone. Microbiology 85 (6): 737-744.
Bruton B.D., 1998. Soilborne diseases in Cucurbitaceae: Pathogen virulence and host resistance. In: Cucurbitaceae '98: evaluation and enhancement of Cucurbit germplasm (J.D. McCreight, ed.), ASHS Press, Alexandria, VA, 143–166.
Bruton B.D., Davis R.M., Gordon T.R., 1995. Occurrence of Acremonium sp. and Monosporascus cannonballus in the major cantaloupe and watermelon growing areas of California. Plant Disease 79: 754.
Bruton B.D., Garcia-Jimenez J., Armengol J., 1999. Analysis of the relationship between temperature and vine declines caused by Acremonium cucurbitacearum and Monosporascus cannonballus. Subtropical Plant Science 51: 23–28.
Bruton B.D., Garcia-Jimenez J., Armengol J., Popham T.W., 2000a. Assessment of virulence of Acremonium cucurbitacearum and Monosporascus cannonballus on Cucumis melo. Plant Disease 84: 907–913.
Bruton B.D., Miller M.E., Garcia-Jimenez J., 1996. Comparison of Acremonium sp. from the Lower Rio Grande Valley of Texas with Acremonium sp. from Spain. Phytopathology 3: 86.
Bruton B.D., Popham T.W., García-Jiménez J., Armengol J., Miller M.E., 2000b. Disease reaction among selected Cucurbitaceae to an Acremonium cucurbitacearum isolate from Texas. Hortscience 35 (4): 677–680.
Carlucci A., Raimondo M. L., Santos J., Phillips A. J. L., 2012. Plectosphaerella species associated with root and collar rots of horticultural crops in southern Italy. Persoonia - Molecular Phylogeny and Evolution of Fungi 28(1): 34–48.
Chabi S.K., Kefela T., Adoukonou-Sagbadja H., Ahoton L., Saidou A., ... Gachomo E. W. 2015. A simple and efficient genomic DNA extraction protocol for large scale genetic analyses of plant biological systems. Plant Gene 1: 43–45.
Chi M.H., Park S.Y., Lee Y.H. 2009. A quick and safe method for fungal DNA extraction. Plant Pathology Journal 25: 108–111.
Chilosi G., Reda R., Aleandri M.P., Camele I., Altieri L., … Frisullo S., 2008. Fungi associated with root rot and collapse of melon in Italy. OEPP/EPPO Bulletin 38: 147–154.
Chowdhury E.H. & Akaike T. 2005. Rapid isolation of high quality, multimeric plasmid DNA using zwitterionic detergent. Journal of Biotechnology 119(4): 343–347. doi: 10.1016/j.jbiotec.2005.05.013
Cluck T., Bruton C., Biles С., 1997. Effect of carbohydrates on chlamydospore production of Acremonium cucurbitacearum. In: Proc. Okla. Acad. Sci. 85th Technical Meeting Oklahoma State University, Academic Science, Oklahoma, USA, 136.
Felsenstein J., 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783–791.
Garrido C., Carbú M., Fernández-Acero F. J., Boonham N., Colyer A., Cantoral J. M., Budge G., 2009. Development of protocols for detection of Colletotrichum acutatum and monitoring of strawberry anthracnose using real-time PCR. Plant Pathology 58: 43–51. doi: 10.1111/j.1365-3059.2008.01933.x
Garsia-Jimenez J., Velazquez M.T., Jorda C., Alfaro-Garcia A., 1994. Acremonium species as the causal agent of muskmelon collapse in Spain. Plant Disease 78: 416–419.
Gubler W.D., 1982. Epidemiology and control of Cephalosporium root and hypocotyl rot of melon. PhD thesis. University of California, Davis, CA, USA.
Gubler W.D., Zitter N.A., Hopkins D.L., Thomas С.Е., 1996. Acremonium hypocotyl rot. In: Compendium of Cucurbit Diseases (T.A. Zitter, D.L. Hopkins, C.E. Thomas, ed.), American Phytopathological Society, St Paul, MN, 9.
Iglesias A., Picó B., Nuez F., 2000. Pathogenicity of fungi associated with melon vine decline and selection strategies for breeding resistant cultivars. Annals of Applied Biology 137: 141–151. doi:10.1111/j.1744-7348.2000.tb00046.x
Jukes T.H., Cantor C.R., 1969. Evolution of protein molecules. In: Mammalian Protein Metabolism (H.N. Munro, ed.), Academic Press, New York, NY, USA, 21-132.
Kumar S., Stecher G., Tamura K., 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33: 1870–1874.
Martinez-Culebras P.V., Abad-Campos P., Garcia-Jimenez J., 2004. Molecular characterization and PCR detection of the melon pathogen Acremonium cucurbitacearum. European Journal of Plant Pathology 110: 801–809.
Mostafa M.A., Attia M.F., Merghany M.M., Salama R.M., 2019. Association of Plectosphaerella melonis with cantaloupe decline for the firsttime in Egypt. Plant Archives 19 (2): 2565–2573.
Perth S.J., 1978. Fundamentals of the cultivation of microorganisms and cells. World, Moscow, Russia, 330 pp (in Russian).
Raimondo M.L., Carlucci A., 2018a. Characterization and pathogenicity of Plectosphaerella spp. collected from basil and parsley in Italy. Phytopathologia Mediterranea 57 (2), 284−295.
Raimondo M.L., Carlucci A., 2018b. Characterization and pathogenicity assessment of Plectosphaerella species associated with stunting disease on tomato and pepper crops in Italy. Plant Pathology 67 (3), 626-641.
Redman R.S., Dunigan D., Rodriguez R.J., 2001. Fungal symbiosis from mutualism to parasitism: who controls the outcome, host or invader? New Phytologist 151: 705–716.
Saitou N., Nei M., 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4: 406–425.
Watts D., MacBeath J.R.E., 2001. Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer. In: DNA Sequencing Protocols. Methods in Molecular Biology. (Graham C.A., Hill A.J.M., eds), Humana Press. vol 167. doi.org/10.1385/1-59259-113-2:153
White T.J., Bruns T.D., Lee S.B., Taylor J.W., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: a Guide to Methods and Applications (M.A. Innis, D.H. Gelfand, J.J. Sninsky, T.J. White, eds.), Academic Press, New York, USA, 315–321.
Alfaro-Fernández A., García-Luis A., 2009. Colonisation and histological changes in muskmelon and autumn squash tissues infected by Acremonium cucurbitacearum or Monosporascus cannonballus. European Journal of Plant Pathology 125 (1): 73–85.
Alfaro-García A., Armengol J., Bruton B.D., Gams W., García-Jiménez J., Martínez-Ferrer G., 1996. The taxonomic position of the causal agent of Acremonium collapse. Mycologia 88: 804–808.
Armengol J., 1997. Aspectos patológicos, epidemiológicos y culturales de Acremonium cucurbitacearum Alfaro-Garcia, W. Gams et J. Garcia-Jiménez. PhD thesis. Universidad Politecnica de Valencia, Valencia, Spain.
Armengol J., Sanz E., Martínez‐Ferrer G., Sales R., Bruton B.D., García‐Jiménez J., 1998. Host range of Acremonium cucurbitacearum, cause of Acremonium collapse of muskmelon. Plant Pathology 47: 29–35.
Biernaki M., Bruton B.D., 2001. Quantitative response of Cucumis melo inoculated with root rot pathogens. Plant Disease 85: 65–70.
Birnboim H.C., Doly J. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7(6): 1513–1523. doi: 10.1093/nar/7.6.1513
Bondarenko S.A., Georgieva M.L., Bilanenko E.N., 2016. Alkalitolerant micromycetes in acidic and neutral soils of the temperate zone. Microbiology 85 (6): 737-744.
Bruton B.D., 1998. Soilborne diseases in Cucurbitaceae: Pathogen virulence and host resistance. In: Cucurbitaceae '98: evaluation and enhancement of Cucurbit germplasm (J.D. McCreight, ed.), ASHS Press, Alexandria, VA, 143–166.
Bruton B.D., Davis R.M., Gordon T.R., 1995. Occurrence of Acremonium sp. and Monosporascus cannonballus in the major cantaloupe and watermelon growing areas of California. Plant Disease 79: 754.
Bruton B.D., Garcia-Jimenez J., Armengol J., 1999. Analysis of the relationship between temperature and vine declines caused by Acremonium cucurbitacearum and Monosporascus cannonballus. Subtropical Plant Science 51: 23–28.
Bruton B.D., Garcia-Jimenez J., Armengol J., Popham T.W., 2000a. Assessment of virulence of Acremonium cucurbitacearum and Monosporascus cannonballus on Cucumis melo. Plant Disease 84: 907–913.
Bruton B.D., Miller M.E., Garcia-Jimenez J., 1996. Comparison of Acremonium sp. from the Lower Rio Grande Valley of Texas with Acremonium sp. from Spain. Phytopathology 3: 86.
Bruton B.D., Popham T.W., García-Jiménez J., Armengol J., Miller M.E., 2000b. Disease reaction among selected Cucurbitaceae to an Acremonium cucurbitacearum isolate from Texas. Hortscience 35 (4): 677–680.
Carlucci A., Raimondo M. L., Santos J., Phillips A. J. L., 2012. Plectosphaerella species associated with root and collar rots of horticultural crops in southern Italy. Persoonia - Molecular Phylogeny and Evolution of Fungi 28(1): 34–48.
Chabi S.K., Kefela T., Adoukonou-Sagbadja H., Ahoton L., Saidou A., ... Gachomo E. W. 2015. A simple and efficient genomic DNA extraction protocol for large scale genetic analyses of plant biological systems. Plant Gene 1: 43–45.
Chi M.H., Park S.Y., Lee Y.H. 2009. A quick and safe method for fungal DNA extraction. Plant Pathology Journal 25: 108–111.
Chilosi G., Reda R., Aleandri M.P., Camele I., Altieri L., … Frisullo S., 2008. Fungi associated with root rot and collapse of melon in Italy. OEPP/EPPO Bulletin 38: 147–154.
Chowdhury E.H. & Akaike T. 2005. Rapid isolation of high quality, multimeric plasmid DNA using zwitterionic detergent. Journal of Biotechnology 119(4): 343–347. doi: 10.1016/j.jbiotec.2005.05.013
Cluck T., Bruton C., Biles С., 1997. Effect of carbohydrates on chlamydospore production of Acremonium cucurbitacearum. In: Proc. Okla. Acad. Sci. 85th Technical Meeting Oklahoma State University, Academic Science, Oklahoma, USA, 136.
Felsenstein J., 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783–791.
Garrido C., Carbú M., Fernández-Acero F. J., Boonham N., Colyer A., Cantoral J. M., Budge G., 2009. Development of protocols for detection of Colletotrichum acutatum and monitoring of strawberry anthracnose using real-time PCR. Plant Pathology 58: 43–51. doi: 10.1111/j.1365-3059.2008.01933.x
Garsia-Jimenez J., Velazquez M.T., Jorda C., Alfaro-Garcia A., 1994. Acremonium species as the causal agent of muskmelon collapse in Spain. Plant Disease 78: 416–419.
Gubler W.D., 1982. Epidemiology and control of Cephalosporium root and hypocotyl rot of melon. PhD thesis. University of California, Davis, CA, USA.
Gubler W.D., Zitter N.A., Hopkins D.L., Thomas С.Е., 1996. Acremonium hypocotyl rot. In: Compendium of Cucurbit Diseases (T.A. Zitter, D.L. Hopkins, C.E. Thomas, ed.), American Phytopathological Society, St Paul, MN, 9.
Iglesias A., Picó B., Nuez F., 2000. Pathogenicity of fungi associated with melon vine decline and selection strategies for breeding resistant cultivars. Annals of Applied Biology 137: 141–151. doi:10.1111/j.1744-7348.2000.tb00046.x
Jukes T.H., Cantor C.R., 1969. Evolution of protein molecules. In: Mammalian Protein Metabolism (H.N. Munro, ed.), Academic Press, New York, NY, USA, 21-132.
Kumar S., Stecher G., Tamura K., 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33: 1870–1874.
Martinez-Culebras P.V., Abad-Campos P., Garcia-Jimenez J., 2004. Molecular characterization and PCR detection of the melon pathogen Acremonium cucurbitacearum. European Journal of Plant Pathology 110: 801–809.
Mostafa M.A., Attia M.F., Merghany M.M., Salama R.M., 2019. Association of Plectosphaerella melonis with cantaloupe decline for the firsttime in Egypt. Plant Archives 19 (2): 2565–2573.
Perth S.J., 1978. Fundamentals of the cultivation of microorganisms and cells. World, Moscow, Russia, 330 pp (in Russian).
Raimondo M.L., Carlucci A., 2018a. Characterization and pathogenicity of Plectosphaerella spp. collected from basil and parsley in Italy. Phytopathologia Mediterranea 57 (2), 284−295.
Raimondo M.L., Carlucci A., 2018b. Characterization and pathogenicity assessment of Plectosphaerella species associated with stunting disease on tomato and pepper crops in Italy. Plant Pathology 67 (3), 626-641.
Redman R.S., Dunigan D., Rodriguez R.J., 2001. Fungal symbiosis from mutualism to parasitism: who controls the outcome, host or invader? New Phytologist 151: 705–716.
Saitou N., Nei M., 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4: 406–425.
Watts D., MacBeath J.R.E., 2001. Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer. In: DNA Sequencing Protocols. Methods in Molecular Biology. (Graham C.A., Hill A.J.M., eds), Humana Press. vol 167. doi.org/10.1385/1-59259-113-2:153
White T.J., Bruns T.D., Lee S.B., Taylor J.W., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: a Guide to Methods and Applications (M.A. Innis, D.H. Gelfand, J.J. Sninsky, T.J. White, eds.), Academic Press, New York, USA, 315–321.