Vol 118, No 2 (Supplement) 2013
Supplement abstract

Vitamin D binding protein-derived macrophage activating factor stimulates proliferation and signalling in a human neuronal cell line

Published January 13, 2014
Keywords
  • Vitamin D,
  • macrophages,
  • vitamin D receptor,
  • vitamin D binding protein-derived macrophage activating factor,
  • human neurons
How to Cite
Morucci, G., Fiore, M. G., Magherini, S., Branca, J. J., Gulisano, M., Thyer, L., Ruggiero, M., & Pacini, S. (2014). Vitamin D binding protein-derived macrophage activating factor stimulates proliferation and signalling in a human neuronal cell line. Italian Journal of Anatomy and Embryology, 118(2), 143. Retrieved from https://oajournals.fupress.net/index.php/ijae/article/view/3808

Abstract

Vitamin D (vitD), vitD binding protein-derived macrophage activating factor (DBP-MAF), and vitD receptor (VDR) are essential for adult neurogenesis [1], and this effect could be responsible for the recently reported effects of DBP-MAF on autism spectrum disorders (ASD) [2]. In order to test this hypothesis, we challenged a human neuronal cell line (SH-SY5Y, IZSLER) with DBP-MAF (Immuno Biotech), and we studied cAMP formation (cAMP EIA kit, Abnova), cell proliferation (MTT assay, Sigma Aldrich), apoptosis (human caspase 3 act, Invitrogen) and cell morphology. SH-SY5Y cells represent a validated in vitro model of human neurons in neurodegenerative diseases [3]. DBP-MAF induced rapid (15 min) formation of cAMP in a dose-dependent manner (0.4-40 ng/ml) as well as increase in cell proliferation at 24-48 and 72 h. Cell morphology was consistent with neurogenesis and an increase in the number of cells with high synthetic activity was observed. No apoptosis following DBP-MAF treatment was observed. Our results open the way to exploit these newly described effects to treat neurodegenerative disorders from Parkinson’s and Alzheimer’s diseases to Myalgic Encephalomyelitis and ASD.