Vol 117, No 2 (Supplement) 2012
Supplement abstract

Magic-F1 transgene cooperates with Pax 3 during early myogenesis to induce muscular hypertrophy

Published 2013-02-21


  • Skeletal muscle hypertrophy,
  • human recombinant protein,
  • FISH analysis,
  • early myogenesis

How to Cite

Ronzoni, F., Ceccarelli, G., Biressi, S., Galli, D., Cassano, M., Benedetti, L., Vercesi, L., Cusella, G., & Sampaolesi, M. (2013). Magic-F1 transgene cooperates with Pax 3 during early myogenesis to induce muscular hypertrophy. Italian Journal of Anatomy and Embryology, 117(2), 166. Retrieved from https://oajournals.fupress.net/index.php/ijae/article/view/4352


Met-Activating Genetically Improved Chimeric Factor-1 (Magic-F1) is a human recombinant protein derived from hepatocyte growth factor/scatter factor (HGF/ SF) and consists in two Met-binding domains repeated in tandem and separated by an artificial linker. It has a reduced affinity for Met and, in contrast to HGF, it elicits activation of the AKT but not the ERK signaling pathway. We recently showed that Magic-F1 induces muscle cell hypertrophy but not progenitor cell proliferation, both in vitro and in vivo where a transgenic mouse express the recombinant protein exclusively in skeletal muscle tissue [1]. Here, we examined the temporal and spatial expression pattern of Magic-F1 in comparison with Pax3 (paired box gene 3) transcription factor during embryogenesis [2]. Ranging from 9.5 to 17.5 dpc (days post coitum) mouse embryos were analyzed by in situ hybridization using whole mounts during early stages of development (9.5-10.5-11.5 dpc) and cryostat sections for later stages (11.5-13.5-15.5-17.5 dpc). We found that Magic-F1 is expressed in developing organs and tissues of mesenchymal origin, where Pax3 signal appears to be downregulated respect to the wt embryos. These data suggest that Magic-F1 could be responsible of muscular hypertrophy, cooperating with Pax3 signal pathway in skeletal muscle precursor cells.