Vol. 120, No. 1 (Supplement) 2015
Supplement abstract

Expression of Trop2 in bladder cancer is modulated by miR125b: in vivo and in vitro analyses

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  • Chiara Avellini,
  • Caterina Licini,
  • Raffaella Lazzarini,
  • Antonio Procopio,
  • Giovanni Muzzonigro,
  • Giovanni Tossetta,
  • Roberta Mazzucchelli,
  • Rosaria Gesuita,
  • Mario Castellucci,
  • Fabiola Olivieri,
  • Daniela Marzioni

Published 2015-09-30

Keywords

  • Bladder cancer,
  • Trop2

How to Cite

Avellini, C., Licini, C., Lazzarini, R., Procopio, A., Muzzonigro, G., Tossetta, G., Mazzucchelli, R., Gesuita, R., Castellucci, M., Olivieri, F., & Marzioni, D. (2015). Expression of Trop2 in bladder cancer is modulated by miR125b: in vivo and in vitro analyses. Italian Journal of Anatomy and Embryology, 120(1), 46. Retrieved from https://oajournals.fupress.net/index.php/ijae/article/view/4000
Copyright Notice

Abstract

Human trophoblastic cell surface antigen 2 (Trop-2) is a 40-kDa transmembrane glycoprotein, first identified as a cell surface marker for human trophoblast cells (1). Elevated expression of Trop-2 has been shown in several types of epithelial cancers and correlated with tumour aggressive and poor prognosis (2-3). The first aim of this study was to evaluate the variation of the Trop-2 expression in normal urothelium and urothelial bladder cancer. The immunohistochemical results showed an increase of Trop-2 levels in bladder cancer tissues with the increase of the severity of the pathology. Recent data identified Trop-2 as a target for miR-125b suggesting a pos sible role of miR-125b in the modulation of Trop-2 protein expression (4). The second aim was to verify if Trop-2 could be a target for miR-125b in bladder cells and to evaluate the possible role of miR-125b in the modulation of Trop-2 protein expression in normal bladder as well as in urothelial bladder cancer. In vitro we showed a contribution of miR-125b in deregulation of Trop-2 protein expression in a bladder cell line and we found that the expression of miR-125b was inversely correlated with the expression of Trop-2 protein on a cohort of bladder cancer tissues. We concluded to investigate in a larger population the use of Trop-2 and/or miR-125b as potential diagnostic markers in urothelial bladder cancer.
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