Vol 118, No 1 (Supplement) 2013
Original Article

A total solid-phase synthesis of DILP8

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  • Sabirov Aydar,
  • Lyu Rong-Ming,
  • Chen Xiang-Qun,
  • Tian Qing,
  • Chang Jaw-Kang

Published 2014-01-11

How to Cite

Aydar, S., Rong-Ming, L., Xiang-Qun, C., Qing, T., & Jaw-Kang, C. (2014). A total solid-phase synthesis of DILP8. Italian Journal of Anatomy and Embryology, 118(1), 13–14. Retrieved from https://oajournals.fupress.net/index.php/ijae/article/view/3199
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Abstract

We have developed a cysteine anchoring method for the synthesis of DILP8 and its analogues. The first is to synthesis of DILP8A SS13-18, C14-MeOBzl, C24-Acm and activate it as DILP8A S13-18, C14-SSPyr C24-Acm. A next step is to synthesize the DILP8BC16-Acm. The desired peptide, DILP8 with Cys(Acm) at A-24 and B-16, was then dissolved in 75% HOAc by addition of Iodine in MeOH and 4M HCl in dioxane. The reaction mixture was monitored by HPLC and the excess iodine was reduced with ascorbic acid. Purification of the peptide was achieved by HPLC. Pure synthetic DILP8 showed a single peak on analytical HPLC with corrected molecular ion. By using the above methods, enough peptide and highly homogenous pure DILP8 were generated.

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