Vol. 129 No. 1 (2025)
Original Article

Corpus callosum motor fibers dissection using Klingler’s method and methylene blue

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  • Paulo Henrique Pires de Aguiar,
  • Carolina Simão Martini,
  • Giulia Bassalo Canals Silva,
  • Giovanna Zambo Galafassi,
  • Jemaila Maciel da Cunha,
  • Giovana Figueira Rodrigues Vieira Pessano,
  • Ricardo Silva Centeno
Paulo Henrique Pires de Aguiar
Federal University of São Paulo, (UNIFESP) São Paulo, Brasil
Carolina Simão Martini
Neurosurgery resident at Santa Paula Hospital, São Paulo, Brazil
Giulia Bassalo Canals Silva
Medical Student at Pontifical Catholic University of São Paulo (PUC/SP), São Paulo, Brazil
Giovanna Zambo Galafassi
Neurosurgery resident at ABC medical school, Santo André, Brazil
Jemaila Maciel da Cunha
Neurosurgery resident at Heliopólis Hospital, São Paulo, Brazil
Giovana Figueira Rodrigues Vieira Pessano
Neurosurgery resident at Heliopólis Hospital, São Paulo, Brazil
Ricardo Silva Centeno
Chief of Epilepsy Department at Federal University of São Paulo (UNIFESP), São Paulo, Brasil
DOI: https://doi.org/10.36253/ijae-15941

Published 2025-07-10

Keywords

  • Lennox Gaustaut Syndrome,
  • corpus callosum,
  • white matter

How to Cite

Pires de Aguiar, P. H., Simão Martini, C., Bassalo Canals Silva, G., Zambo Galafassi, G., Maciel da Cunha, J., Figueira Rodrigues Vieira Pessano, G., & Silva Centeno, R. (2025). Corpus callosum motor fibers dissection using Klingler’s method and methylene blue. Italian Journal of Anatomy and Embryology, 129(1), 47–53. https://doi.org/10.36253/ijae-15941

Copyright (c) 2025 Paulo Henrique Pires de Aguiar, Carolina Simão Martini, Giulia Bassalo Canals Silva, Giovanna Zambo Galafassi, Jemaila Maciel da Cunha, Giovana Figueira Rodrigues Vieira Pessano, Ricardo Silva Centeno

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Background: The corpus callosum (CC) is the largest white matter bundle in the brain, connecting the left and right cerebral hemispheres. It plays a crucial role in integrating information and facilitating somatosensory, motor, and cognitive function. This study examined the anatomy of the CC using white matter dissection and explored a posterior callosotomy approach for treating drop attacks in Lennox-Gastaut syndrome (LGS). LGS is a rare form of childhood-onset epilepsy, characterized by multiple seizure types, abnormal electroencephalograms, and progressive mental retardation. The authors proposed that fibers from the medial and posterior CC regions converge on the precentral gyrus, and dissection was performed to investigate this theory. Methods: This study employed the Klingler method to examine white matter fibers in four adult brains. Dissection involved removing the cortex from the superior frontal, precentral, and postcentral gyri, followed by the cingulate gyrus. Results: The CC is divided into five parts: the rostrum, genu, body, isthmus, and splenium. Each part has distinct anatomical relationships with the lateral ventricles and connects to different cortical regions Conclusions: Our findings are consistent with the evidence that the motor fibers of the brain traverse the posterior region of the corpus callosum, suggesting that selective posterior callosotomy may be a more effective treatment for refractory drop attacks in Lennox-Gastaut Syndrome.

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