Vol. 123, No. 1 (Supplement) 2018
Supplement abstract

Molecular chaperones expression levels and localization in non-tumoral and tumoral thyroid tissues

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  • Francesca Rappa,
  • Celeste Caruso Bavisotto,
  • Antonella Marino Gammazza,
  • Fabio Bucchieri,
  • Everly Conway de Macario,
  • Alberto J.L. Macario,
  • Calogero Cipolla,
  • Giovanni Tomasello,
  • Francesco Carini,
  • Daniela Cabibi,
  • Claudia Campanella,
  • Felicia Farina,
  • Francesco Cappello
Francesca Rappa
Section of Human Anatomy, Department of Experimental Biomedicine and Clinical Neuroscience, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy
Celeste Caruso Bavisotto
Section of Human Anatomy, Department of Experimental Biomedicine and Clinical Neuroscience, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy
Antonella Marino Gammazza
Section of Human Anatomy, Department of Experimental Biomedicine and Clinical Neuroscience, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy
Fabio Bucchieri
Section of Human Anatomy, Department of Experimental Biomedicine and Clinical Neuroscience, University of Palermo, Palermo, Italy
Everly Conway de Macario
Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore- Institute of Marine and Environmental Technology (IMET), Baltimore, Maryland, USA
Alberto J.L. Macario
Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore- Institute of Marine and Environmental Technology (IMET), Baltimore, Maryland, USA
Calogero Cipolla
Department of Oncology, Division of General and Oncological Surgery, University of Palermo, Palermo, Italy
Giovanni Tomasello
Section of Human Anatomy, Department of Experimental Biomedicine and Clinical Neuroscience, University of Palermo, Palermo, Italy
Francesco Carini
Section of Human Anatomy, Department of Experimental Biomedicine and Clinical Neuroscience, University of Palermo, Palermo, Italy
Daniela Cabibi
Pathology Institute, Department BG. D’Alessandro^, University of Palermo, Palermo, Italy
Claudia Campanella
Section of Human Anatomy, Department of Experimental Biomedicine and Clinical Neuroscience, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy
Felicia Farina
Section of Human Anatomy, Department of Experimental Biomedicine and Clinical Neuroscience, University of Palermo, Palermo, Italy
Francesco Cappello
Section of Human Anatomy, Department of Experimental Biomedicine and Clinical Neuroscience, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy
DOI: https://doi.org/10.13128/ijae-11500

Published 2018-12-30

Keywords

  • Papillary thyroid carcinoma,
  • exosome,
  • hsp

How to Cite

Rappa, F., Caruso Bavisotto, C., Marino Gammazza, A., Bucchieri, F., Conway de Macario, E., Macario, A. J., Cipolla, C., Tomasello, G., Carini, F., Cabibi, D., Campanella, C., Farina, F., & Cappello, F. (2018). Molecular chaperones expression levels and localization in non-tumoral and tumoral thyroid tissues. Italian Journal of Anatomy and Embryology, 123(1), 179. https://doi.org/10.13128/ijae-11500
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Abstract

Papillary thyroid carcinoma (PTC) is the most frequently occurring subtype of thyroid can- cer. Exosomes (EXs) secreted from cells to the extracellular environment play an important role in intercellular communication in normality and pathology. Recent data indicates that tumor cells-derived EXs contribute to cancer progression through the modulation of tumor microen- vironment [1]. Heat Shock Protein (HSPs) are often overexpressed during carcinogenesis and different studies shown that they can be released by tumors cells and that the mechanism of release is mediated by EXs pathway. In this project we performed an immunomorphological study to investigate Hsp60, 90,70,27 levels expression profile in thyroid tissue from patients with benign goiter (used as benign desease) and patients with PTC. Moreover for each patient, blood samples were collected before and a one week after surgery, to obtain EXs. We performed Western Blotting analysis to verify the presence and the levels of the same HSPs. The immu- noistochemistry shown an overexpression of Hsp60,90 and 27 in the PTC cases comparison with peritumoral tissue and with goiter cases. Instead the Hsp70 levels showed no significant changes. In particular Hsp60, 90 and 27 were visible at cytoplasmic and membrane levels. Data regarding exosomal fraction assessment by standard methods (TEM, and WB analysis for Alix) to identify exosomes confirmed their identity. The levels of Hsp60, 90,27 in the exosomes of patients with PTC before surgery were significantly higher than in the exosomes from the same patients after surgery. The data obtained shown that, as demonstrated in other cancer type [2], the HSP levels studied increased in PTC specimens respect to goiter specimens. Moreover the membrane localization of these HSP suggested a their release in tumor microenviroment, in fact we observed exosomal HSP before surgery in PTC patients. The HSP decreases after surgery indicated that if disease recurrence occurs, HSP levels will increase again. For this reason we hipotized that chaperonins could be good candidates as biomarkers of PTC.

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